• Mucosal tissue specificity of...

*Result*: Mucosal tissue specificity of PAMP-related PRR gene expression in Scophthalmus maximus under different modes of Vibrio anguillarum stimulation.

Title:
Mucosal tissue specificity of PAMP-related PRR gene expression in Scophthalmus maximus under different modes of Vibrio anguillarum stimulation.
Authors:
Gou D; State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Qingdao), Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266237, China., Sun Z; State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Qingdao), Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266237, China. Electronic address: sunzb@ysfri.ac.cn., Wang X; State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Qingdao), Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266237, China., Ma A; State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Qingdao), Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266237, China. Electronic address: maaj@ysfri.ac.cn., Huang Z; State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Qingdao), Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266237, China., Sun W; State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Qingdao), Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266237, China., Liu Z; State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Qingdao), Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266237, China., Tian M; State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Qingdao), Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266237, China., Gao Y; State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Qingdao), Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266237, China.
Source:
Fish & shellfish immunology [Fish Shellfish Immunol] 2026 Jan; Vol. 168, pp. 110945. Date of Electronic Publication: 2025 Oct 16.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Academic Press Country of Publication: England NLM ID: 9505220 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9947 (Electronic) Linking ISSN: 10504648 NLM ISO Abbreviation: Fish Shellfish Immunol Subsets: MEDLINE
Imprint Name(s):
Original Publication: London ; New York : Academic Press, c1991-
MeSH Terms:
Fish Diseases*/immunology , Fish Diseases*/microbiology , Fish Diseases*/genetics , Flatfishes*/immunology , Flatfishes*/genetics , Vibrio Infections*/immunology , Vibrio Infections*/veterinary , Vibrio Infections*/genetics , Vibrio Infections*/microbiology , Fish Proteins*/genetics , Fish Proteins*/immunology , Fish Proteins*/metabolism , Receptors, Pattern Recognition*/genetics , Receptors, Pattern Recognition*/immunology , Receptors, Pattern Recognition*/metabolism , Immunity, Innate*/genetics , Gene Expression Regulation*/immunology , Immunity, Mucosal*/genetics, Vibrio/physiology ; Lipopolysaccharides/pharmacology ; Pathogen-Associated Molecular Pattern Molecules/immunology ; Mucous Membrane/immunology ; Animals ; Organ Specificity
Contributed Indexing:
Keywords: GGE; Gram-negative bacteria; Mucosal immunity; Pattern recognition receptors
Substance Nomenclature:
0 (Fish Proteins)
0 (Lipopolysaccharides)
0 (Receptors, Pattern Recognition)
0 (Pathogen-Associated Molecular Pattern Molecules)
SCR Organism:
Vibrio anguillarum
Entry Date(s):
Date Created: 20251018 Date Completed: 20251129 Latest Revision: 20251129
Update Code:
20260130
DOI:
10.1016/j.fsi.2025.110945
PMID:
41109540
Database:
MEDLINE

*Further Information*

*To investigate the immune responses of pathogen-associated molecular patterns (PAMPs) recognition-receptor genes in Scophthalmus maximus stimulated by the Gram-negative bacterium live/inactivated Vibrio anguillarum, and lipopolysaccharide (LPS), skin, gill, and intestinal tissues of fish at 0-96 h post-invasion were collected. mRNA was extracted from these mucosal tissues, and the gene expression of the following 7 PAMP recognition receptors was quantitatively analyzed using real-time fluorescence quantitative PCR: LEC, SAP, TLR2, TLR5, TLR14, PGRP2, and LIMP-2. Expression of SmTLR2, SmLEC, SmSAP, and SmPGRP2 were ubiquitously induced in mucosal tissues through invasion by V. anguillarum and related bacterial components. Under V. anguillarum stimulation, SmTLR2, SmTLR14, SmSAP, and SmPGRP2 exhibited the fastest expression in the skin. Under LPS stimulation, SmLEC showed the most rapid upregulation in the gills. Under inactivated V. anguillarum stimulation, the earliest expression of SmLIMP-2 occurred in the intestine. Peak expression under live/inactivated V. anguillarum, and LPS stimulation occurred at 24-48 h, 12-96 h, and 12-96 h post-immunization, reaching values that were 198.91-1.49-fold, 2.3-202.46-fold, and 2.27-96.10-fold higher than those of the control group, respectively. These results demonstrated that pattern recognition receptor (PRR) genes in turbot mucosal tissues were rapidly and abundantly expressed to mediate immune responses post-immunostimulation. We applied Genotype plus Genotype × Environment Interaction (GGE) biplot analysis to comprehensively evaluate immunostimulants and PRR genes regulation and then used the results to establish an optimal stimulation strategy for selective breeding against bacterial diseases. SmTLR14, SmPGRP2, SmLEC, and SmTLR2 were identified as optimal indicator genes in mucosal tissues following stimulation by live/inactivated V. anguillarum or LPS.
(Copyright © 2025 Elsevier Ltd. All rights reserved.)*

*Competing interests The authors have declared that no competing interest exists.*