*Result*: Microbiological and molecular profile of furcation defects in a population with untreated periodontitis.

Title:
Microbiological and molecular profile of furcation defects in a population with untreated periodontitis.
Authors:
Santamaria, Pasquale, Jin, Yi, Ghuman, Mandeep, Shoaie, Saeed, Spratt, David, Troiano, Giuseppe, Nibali, Luigi
Source:
Journal of Clinical Periodontology; Nov2024, Vol. 51 Issue 11, p1421-1432, 12p
Subject Terms:
GINGIVA, PERIODONTAL disease, MICROBIOTA, TISSUE inhibitors of metalloproteinases, RNA, GENES, EPIDERMAL growth factor, DENTAL plaque, CLUSTER sampling, PROTEOLYTIC enzymes, EXUDATES & transudates, IMMUNOASSAY, PERIODONTITIS, BIOMARKERS, INTERLEUKINS
Geographic Terms:
ITALY
Database:
Complementary Index

*Further Information*

*Aim: To describe the microbiological composition of subgingival dental plaque and molecular profile of gingival crevicular fluid (GCF) of periodontal furcation‐involved defects. Materials and Methods: Fifty‐seven participants with periodontitis contributed with a degree II–III furcation involvement (FI), a non‐furcation (NF) periodontal defect and a periodontally healthy site (HS). Subgingival plaque was analysed by sequencing the V3–V4 region of the 16S rRNA gene, and a multiplex bead immunoassay was carried out to estimate the GCF levels of 18 GCF biomarkers. Aiming to explore inherent patterns and the intrinsic structure of data, an AI‐clustering method was also applied. Results: In total, 171 subgingival plaque and 84 GCF samples were analysed. Four microbiome clusters were identified and associated with FI, NF and HS. A reduced aerobic microbiota (p =.01) was detected in FI compared with NF; IL‐6, MMP‐3, MMP‐8, BMP‐2, SOST, EGF and TIMP‐1 levels were increased in the GCF of FI compared with NF. Conclusions: This is the first study to profile periodontal furcation defects from a microbiological and inflammatory standpoint using conventional and AI‐based analyses. A reduced aerobic microbial biofilm and an increase of several inflammatory, connective tissue degradation and repair markers were detected compared with other periodontal defects. [ABSTRACT FROM AUTHOR]

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